Sarcoma

Table I : cell lines with wt p53

Cell line	ATCC		Reference
FPBH		Ewing sarcoma
SAL-2		Ewing sarcoma
STA-ET-1		Ewing sarcoma
WE-68		Ewing sarcoma
VH-64		Ewing sarcoma
STA-ET-6		Ewing sarcoma
TC 252		Ewing sarcoma
U2OS*	HTB-96	Osteosarcoma
SJSA**	CRL-2098	Osteosarcoma
HT1080	CCL-121	Fibosarcoma
A-204		Undifferentiated sarcoma

           

* Mdm2 overexpression
**Mdm2 amplification

Table II : cell lines with p53 gene deletion or rearrangement

Cell line	ATCC		Reference
Saos-2	HTB-85	Osteosarcoma
SK-N-MC	HTB-10	Ewing sarcoma

Table III : cell lines with p53 splice mutation
(exonic mutations that modify splice are listed in table IV)

no data

Table IV: cell lines with p53 mutations (missense or frameshift)
More information is available in the handbook

Pos: Codon position (1 to 393);
WT: Normal base sequence of the codon in which the mutation occurred;
Mut Sequence of the mutated codon. If the mutation is a deletion or an insertion, it is indicated by "del" or "ins" followed by the number of deleted or inserted bases. The position in the codon is indicated by “a”, “b”, or “c” for the first, second or third base of the codon respectively. Example: "del66b" is a deletion of 66 bases including the second base of the codon; "ins4a" is an insertion of 4 bases occurring between the first and the second base of the codon. Label a, b, or c is omitted if the boundary of the deletion or insertion is unknown
AA: Wild type amino acid;
Mut: Mutant amino acid. Stop: nonsense mutation: Fs.: Frameshift mutation: InF: In-frame insertion or deletion;
Comp: complexity of the mutation: SM: Single mutational event in the tumor; DMU (Double Mutation Unknown): Two p53 mutations in the same tumor but their allelique distribution is unknown; DMD (Double Mutation Different allele): Two p53 mutations in the same tumor on two different p53 alleles; DMS (Double Mutation Same allele): Two p53 mutations in the same tumor on the same p53 allele: MM (Multiple Mutation): More than two p53 mutations in the same tumor;
NB: Number of tumors with this particular mutant in the database;
Ref: reference number;
Comments: information;

Table of references (available also in the handbook)

Pos.	WT	Mut	AA	Mut	Comp	Name	NB	Ref	Comments
Angiosarcoma (hepatic)
179	CAT	CTT	His	Leu	SM	ASL5	38	1353	Single report
Chondrosarcoma
175	CGC	CAC	Arg	His	SM	CH-1	1187	1373	Single report
Ewing's Sarcoma
194	CTT	CGT	Leu	Arg	SM	19	66	328	Single report
175	CGC	CAC	Arg	His	SM	58	1187	328	Single report
176	TGC	TTC	Cys	Phe	SM	59	191	328	Single report
273	CGT	TGT	Arg	Cys	SM	63	687	328	Single report
285	GAG	AAG	Glu	Lys	SM	64	165	328	Single report
118	ACA	ins2	Thr	Fs.	SM	A673	1	226	wt in COSMIC
283	CGC	DEL1C	Arg	Fs.	SM	ES-1-OT	4	245	Single report
181	CGC	CAC	Arg	His	SM	EW-13	34	2249	Mutation in COSMIC database
164	AAG	GAG	Lys	Glu	SM	EW-24	25	2249	Mutation in COSMIC database
273	CGT	TGT	Arg	Cys	SM	IARC-EW2	687	226	Single report
273	CGT	TGT	Arg	Cys	SM	RD-ES	687	226	Confirmed in another publication
273	CGT	CAT	Arg	His	SM	RM 82	780	226	Single report
176	TGC	TTC	Cys	Phe	SM	SCMC-ES-2	191	245	Single report
141	TGC	TAC	Cys	Tyr	SM	SCMCS-ES-1	103	245	Single report
176	TGC	TTC	Cys	Phe	SM	SK-ES-1	191	226	Confirmed in another publication
176	TGC	TTC	Cys	Phe	SM	SK-PN-DW	191	2249	Mutation in COSMIC database
273	CGT	TGT	Arg	Cys	SM	SMB	687	226	Single report
277	TGT	TAT	Cys	Tyr	SM	STA-ET-2.1	28	226	Single report
273	CGT	TGT	Arg	Cys	SM	STA-ET-7.1	687	226	Single report
176	TGC	TTC	Cys	Phe	SM	W-ES	191	245	Single report
Fibrosarcoma
213	CGA	TGA	Arg	Stop	SM	SW684	306	2249	Mutation in COSMIC database
Leyomyosarcoma
215	AGT	ins1	Ser	Fs.	SM	LMS6-93	3	1552	Single report
237	ATG	AAG	Met	Lys	DMU	SK-LMS-1	11	2198	Controversy with other publications
245	GGC	AGC	Gly	Ser	SM	SK-LMS-1	440	14	Controversy with other publications
245	GGC	AGC	Gly	Ser	DMU	SK-LMS-1	440	2198	Controversy with other publications
175	CGC	CAC	Arg	His	DMU	SK-UT-1	1187	2249	Controversy with other publications
175	CGC	CAC	Arg	His	SM	SK-UT-1	1187	14	Controversy with other publications
248	CGG	CAG	Arg	Gln	DMU	SK-UT-1	883	2249	Controversy with other publications
Liposarcoma
251	ATC	AAC	Ile	Asn	SM	SW872	21	2249	Mutation in COSMIC database
Osteosarcoma
156	CGC	CCC	Arg	Pro	SM	HOS	38	25	Confirmed in another publication
306	CGA	TGA	Arg	Stop	SM	HOSM-2	160	1920	Single report
76	GCA	ins1a	Ala	Fs.	SM	HuO-3N1	1	2249	Mutation in COSMIC database
286	GAA	AAA	Glu	Lys	SM	OH	86	272	Single report
250	CCC	del8	Pro	Fs.	SM	RIT-1B	1	65	Single report
245	GGC	AGC	Gly	Ser	SM	RIT2	440	65	Single report
Rhabdomyosarcoma
219	CCC	del4	Pro	Fs.	SM	ctr	1	97	Single report
248	CGG	TGG	Arg	Trp	SM	RD	728	14	Confirmed in another publication. wt in COSMIC
273	CGT	TGT	Arg	Cys	SM	Rh30	687	1190	Controversy with other publications
280	AGA	AGT	Arg	Ser	SM	RH30	14	97	Controversy with other publications
156	CGC	CCC	Arg	Pro	SM	RMS	38	14	Single report
Sarcoma
316	CCC	CCT	Pro	Pro	SM	GCT	6	2249	Mutation in COSMIC database
317	CAG	TAG	Gln	Stop	SM	GCT	25	2249	Mutation in COSMIC database
326	GAA	TAA	Glu	Stop	SM	S-117	9	2249	Mutation in COSMIC database