Hepatocellular carcinoma

Table I : cell lines with wt p53

Cell line	ATCC	Reference
HuH-6 clone 5		1006
HuH-1*		1042
HCC-M*		1042
SK-Hep1	HTB-52	1042
HepG2	HB-8065	1042
WRL 68		230
FOCUS*,**		232

*HBV positive

Table II : cell lines with p53 gene deletion or rearrangement

Cell line	ATCC	Reference
Hep3B*	HB-8064

*HBV positive

Table III : cell lines with p53 splice mutation
(exonic mutations that modify splice are listed in table IV)

No data

Table IV: cell lines with p53 mutations (missense or frameshift)
More information is available in the handbook

Pos: Codon position (1 to 393);
WT: Normal base sequence of the codon in which the mutation occurred;
Mut Sequence of the mutated codon. If the mutation is a deletion or an insertion, it is indicated by "del" or "ins" followed by the number of deleted or inserted bases. The position in the codon is indicated by “a”, “b”, or “c” for the first, second or third base of the codon respectively. Example: "del66b" is a deletion of 66 bases including the second base of the codon; "ins4a" is an insertion of 4 bases occurring between the first and the second base of the codon. Label a, b, or c is omitted if the boundary of the deletion or insertion is unknown
AA: Wild type amino acid;
Mut: Mutant amino acid. Stop: nonsense mutation: Fs.: Frameshift mutation: InF: In-frame insertion or deletion;
Comp: complexity of the mutation: SM: Single mutational event in the tumor; DMU (Double Mutation Unknown): Two p53 mutations in the same tumor but their allelique distribution is unknown; DMD (Double Mutation Different allele): Two p53 mutations in the same tumor on two different p53 alleles; DMS (Double Mutation Same allele): Two p53 mutations in the same tumor on the same p53 allele: MM (Multiple Mutation): More than two p53 mutations in the same tumor;
NB: Number of tumors with this particular mutant in the database;
Ref: reference number;
Comments: information;

Table of references (available also in the handbook)

Pos.	WT	Mut	AA	Mut	Comp	Name	NB	Ref	Comments
281	GAC	CAC	Asp	His	SM	OHR*	41	589	Single report
139	AAG	GAG	Lys	Glu	SM	HCC-T	3	1042	Single report
249	AGG	AGT	Arg	Ser	SM	HCKI-1	389	1356	Single report
244	GGC	GCC	Gly	Ala	SM	HLE	10	1069	Controversy with other publications
249	AGG	AGC	Arg	Ser	SM	HLE	34	230	Controversy with other publications
272	GTG	ATG	Val	Met	SM	HLE	105	1006	Controversy with other publications
244	GGC	GCC	Gly	Ala	SM	HLF	10	230	Confirmed in two other publications
264	CTA	del18	Leu	InF	SM	HuH-4	1	230	Single report
220	TAT	TGT	Tyr	Cys	SM	HuH-7	336	230	wt in another publication
249	AGG	AGT	Arg	Ser	SM	Malhavu	389	232	Single report
249	AGG	AGT	Arg	Ser	SM	PLC/PRF/5	389	38	Confirmed in two other publications
164	AAG	TAG	Lys	Stop	SM	SNU-387	18	2249	Mutation in COSMIC database
161	GCC	ACC	Ala	Thr	SM	SNU-449	75	2249	Mutation in COSMIC database
262	GGT	GAT	Gly	Asp	SM	SNU-475	6	2249	Mutation in COSMIC database
200	AAT	AAA	Asn	Lys	SM	TONG/HCC	1	1042	wt in another publication

* Hepatoblastoma