Non Small Cell Lung Cancer

Table I : cell lines with wt p53

Cell line	ATCC	Ref.	essssssss	Cell line	ATCC	Ref.
A-549	CCL-185	1006		NCI-H1944	CRL-5907	678
LU99		1006		NCI-H2023	CRL-5912	678
LU99A		1006		NCI-H2030	CRL-5914	678
LU99B		1006		NCI-H2077	CRL-5919	678
A427	HTB-53	1382		NCI-H2126	CRL-5925	678
Ma-12		1382		NCI-H2347	CRL-5942	678
Ma-17		1382		NCI-H1395	CRL-5868	2249
Ma-26		1382		NCI-H1563	CRL-5875	2249
NCI-H460	HTB-177	678		NCI-H1650	CRL-5883	678
NCI-H726		678		NCI-H1666	CRL-5885	678
NCI-H838	CRL-5844	678		NCI-H1975	CRL-5908	2249
NCI-H1385	CRL-5867	678		NCI-H2170	CRL-5928	2249
NCI-H1568	CRL-5876	678		NCI-H2228	CRL-5935	2249
NCI-H1570		678		NCI-H2342	CRL-5941	2249
NCI-H1653		678		NCI-H2347	CRL-5970	2249
NCI-H1725		678		NCI-H810	CRL-5816	678

Table II : cell lines with p53 gene deletion or rearrangement

Cell line	ATCC	Reference
NCI-H1299	CRL-5803
H-358	CRL5807
Calu-1	HTB-54

Table III : cell lines with p53 splice mutation
(exonic mutations that modify splice are listed in table IV)

Cell line	ATCC	Mutation	reference
NCI-H648	CRL-5834	intron7	92
NCI-H1710		intron8	92
NCI-H920	CRL-5850	intron5	92
NCI-H1792	CRL-5895	c.672+1G>A	92

Table IV: cell lines with p53 mutations (missense or frameshift)
More information is available in the handbook

Pos: Codon position (1 to 393);
WT: Normal base sequence of the codon in which the mutation occurred;
Mut Sequence of the mutated codon. If the mutation is a deletion or an insertion, it is indicated by "del" or "ins" followed by the number of deleted or inserted bases. The position in the codon is indicated by “a”, “b”, or “c” for the first, second or third base of the codon respectively. Example: "del66b" is a deletion of 66 bases including the second base of the codon; "ins4a" is an insertion of 4 bases occurring between the first and the second base of the codon. Label a, b, or c is omitted if the boundary of the deletion or insertion is unknown
AA: Wild type amino acid;
Mut: Mutant amino acid. Stop: nonsense mutation: Fs.: Frameshift mutation: InF: In-frame insertion or deletion;
Comp: complexity of the mutation: SM: Single mutational event in the tumor; DMU (Double Mutation Unknown): Two p53 mutations in the same tumor but their allelique distribution is unknown; DMD (Double Mutation Different allele): Two p53 mutations in the same tumor on two different p53 alleles; DMS (Double Mutation Same allele): Two p53 mutations in the same tumor on the same p53 allele: MM (Multiple Mutation): More than two p53 mutations in the same tumor;
NB: Number of tumors with this particular mutant in the database;
Ref: reference number;
Comments: information;

Table of references (available also in the handbook)

Pos.	WT	Mut	AA	Mut	Comp	Name	NB	Ref	Comments
229	TGT	TGA	Cys	Stop	SM	866MT	6	16	Single report
278	CCT	TCT	Pro	Ser	SM	ABC-1	87	1382	Single report
71	CCC	del1	Pro	Fs.	SM	ACC-LC-319	2	1976	Single report
281	GAC	GGC	Asp	Gly	SM	ACC-LC94	16	1976	Single report
209	AGA	TGA	Arg	Stop	SM	CAEP	14	1393	Single report
135	TGC	TTC	Cys	Phe	SM	CAL-12T	52	2249	Mutation in COSMIC database
280	AGA	AAA	Arg	Lys	SM	Ca-Lu-1	78	1081	Single report
237	ATG	ATT	Met	Ile	SM	CALU-3	52	2249	Mutation in COSMIC database
196	CGA	TGA	Arg	Stop	SM	CALU6	241	16	Single report
175	CGC	CAC	Arg	His	SM	CMRC-LCD	1187	1382	Single report
171	GAG	TAG	Glu	Stop	SM	EBC-1	25	1382	Single report
187	GGT	del111	Gly	InF	SM	EKVX	1	1018	Controversy with other publications
203	GTG	GTT	Val	Val	SM	EKVX	2	2249	Controversy with other publications
204	GAG	TAG	Glu	Stop	SM	EKVX	46	2249	Controversy with other publications
273	CGT	TGT	Arg	Cys	SM	GLCA2	687	1386	Single report
248	CGG	CTG	Arg	Leu	DMU	GLCP1	124	1386	Single report
349	GAA	TAA	Glu	Stop	DMU	GLCP1	7	1386	Single report
139	AAG	del1c	Lys	Fs.	SM	HCC1438	1	1327	Single report
154	GGC	GTC	Gly	Val	SM	HCC2108	65	1327	Single report
234	TAC	TGC	Tyr	Cys	SM	HCC2279	133	1327	Single report
175	CGC	CTC	Arg	Leu	SM	HOP-92	25	1018	Confirmed in COSMIC database
132	AAG	AAT	Lys	Asn	SM	IGR-Heu	23	2195	Single report
249	AGG	AGT	Arg	Ser	SM	KNS-62	389	2249	Mutation in COSMIC database
237	ATG	ATT	Met	Ile	SM	LC-1-SQ	52	364	Confirmed in another publication
241	TCC	TGC	Ser	Cys	SM	LC-2-ad	36	2249	Mutation in COSMIC database
216	GTG	TTG	Val	Leu	SM	LCLC-103H	13	2249	Mutation in COSMIC database
248	CGG	CTG	Arg	Leu	SM	LCMS	124	1382	Single report
272	GTG	ATG	Val	Met	SM	LK-2	105	1006	Single report
11	GAG	CAG	Glu	Gln	SM	LU-65	10	1382	Single report
273	CGT	CAT	Arg	His	SM	LX-1	780	1081	Single report
248	CGG	TGG	Arg	Trp	SM	LXF-289	728	2249	Mutation in COSMIC database
248	CGG	CAG	Arg	Gln	SM	MA1	883	1382	Confirmed in another publication
245	GGC	GTC	Gly	Val	SM	MA-10	84	1382	Single report
282	CGG	TGG	Arg	Trp	SM	MA14	600	1574	Single report
175	CGC	CAC	Arg	His	SM	MA2	1187	1382	Confirmed in another publication
337	CGC	TGC	Arg	Cys	SM	MA-24	19	1382	Single report
237	ATG	ATA	Met	Ile	SM	MA25	123	1382	Confirmed in another publication
121	TCT	del1c	Ser	Fs.	SM	MA-29	1	1382	Single report
245	GGC	TGC	Gly	Cys	SM	MA3	86	1382	Confirmed in another publication
245	GGC	GTC	Gly	Val	SM	MA36	84	1574	Single report
273	CGT	CAT	Arg	His	SM	NCI-H1155	780	92	Confirmed in two other publications
239	AAC	ins1b	Asn	Fs.	SM	NCI-H125	1	678	Single report
298	GAG	TAG	Glu	Stop	SM	NCI-H1264	71	92	Single report
248	CGG	TGG	Arg	Trp	SM	NCI-H1284	728	678	Single report
234	TAC	TGC	Tyr	Cys	SM	NCI-H1304	133	678	wt in COSMIC
224	GAG	GAC	Glu	Asp	SM	NCI-H1334	10	92	Single report
285	GAG	AAG	Glu	Lys	SM	NCI-H1355	165	92	Confirmed in another publication
47	CCG	CTG	Pro	Leu	SM	NCI-H1373	3	106	Controversy with other publications
144	CAG	TAG	Gln	Stop	SM	NCI-H1404	53	92	Single report
141	TGC	TGG	Cys	Trp	SM	NCI-H1435	11	92	Single report
267	CGG	CCG	Arg	Pro	SM	NCI-H1437	19	92	Confirmed in another publication
89	CCC	del1	Pro	Fs.	SM	NCI-H1466	3	92	Single report
34	CCC	ins1	Pro	Fs.	DMU	NCI-H157	1	1382	Controversy with other publications
282	CGG	CCG	Arg	Pro	SM	NCI-H157	21	2021	Controversy with other publications
298	GAG	TAG	Glu	Stop	SM	NCI-H157	71	92	Controversy with other publications
298	GAG	TAG	Glu	Stop	DMU	NCI-H157	71	1382	Controversy with other publications
248	CGG	CTG	Arg	Leu	SM	NCI-H1573	124	92	Single report
144	CAG	TAG	Gln	Stop	SM	NCI-H1581	53	92	wt in COSMIC
273	CGT	CTT	Arg	Leu	SM	NCI-H1623	147	92	Single report
175	CGC	CAC	Arg	His	SM	NCI-H1628	1187	678	Single report
35	TTG	ins1b	Leu	Fs.	SM	NCI-H1648	1	92	wt in COSMIC
176	TGC	TAC	Cys	Tyr	SM	NCI-H1651	88	92	Single report
285	GAG	AAG	Glu	Lys	SM	NCI-H1703	165	92	Controversy with other publications
273	CGT	CTT	Arg	Leu	SM	NCI-H1734	147	92	wt in COSMIC
242	TGC	TTC	Cys	Phe	SM	NCI-H1755	88	92	Single report
247	AAC	AAT	Asn	Asn	SM	NCI-H1770	39	2249	Mutation in COSMIC database
248	CGG	TGG	Arg	Trp	SM	NCI-H1770	728	2249	Mutation in COSMIC database
157	GTC	TTC	Val	Phe	SM	NCI-H1781	177	92	Single report
209	AGA	TGA	Arg	Stop	SM	NCI-H1793	14	92	Controversy with other publications
273	CGT	CAT	Arg	His	SM	NCI-H1793	780	2249	Controversy with other publications
273	CGT	CTT	Arg	Leu	SM	NCI-H1838	147	2249	Mutation in COSMIC database
237	ATG	ATA	Met	Ile	SM	NCI-H1869	123	678	Single report
209	AGA	TGA	Arg	Stop	SM	NCI-H1904	14	92	Single report
291	AAG	TAG	Lys	Stop	SM	NCI-H1915	9	92	Single report
242	TGC	TGG	Cys	Trp	SM	NCI-H1993	16	2249	Mutation in COSMIC database
273	CGT	CTT	Arg	Leu	SM	NCI-H2009	147	92	Single report
262	GGT	GTT	Gly	Val	SM	NCI-H2030	14	2249	Mutation in COSMIC database
242	TGC	TGG	Cys	Trp	SM	NCI-H2073	16	92	Single report
220	TAT	TCT	Tyr	Ser	SM	NCI-H2086	20	106	Single report
157	GTC	TTC	Val	Phe	SM	NCI-H2087	177	92	Confirmed in another publication
248	CGG	TGG	Arg	Trp	SM	NCI-H2106	728	106	Single report
16	CAG	CTG	Gln	Leu	DMU	NCI-H2122	1	2249	Mutation in COSMIC database
176	TGC	TTC	Cys	Phe	DMU	NCI-H2122	191	2249	Mutation in COSMIC database
62	GAA	TAA	Glu	Stop	SM	NCI-H2126	8	2249	Mutation in COSMIC database
161	GCC	ACC	Ala	Thr	SM	NCI-H2250	75	92	Single report
158	CGC	CTC	Arg	Leu	SM	NCI-H226	92	92	Controversy with other publications
309	CCC	GCC	Pro	Ala	SM	NCI-H226	1	1018	Controversy with other publications
154	GGC	GTC	Gly	Val	SM	NCI-H2291	65	2249	Mutation in COSMIC database
246	ATG	ATC	Met	Ile	SM	NCI-H23	6	17	Confirmed in another publication
273	CGT	CAT	Arg	His	SM	NCI-H2405	780	2249	Mutation in COSMIC database
248	CGG	CTG	Arg	Leu	SM	NCI-H322	124	92	Confirmed in another publication
249	AGG	AGC	Arg	Ser	SM	NCI-H324	34	678	Controversy with other publications
249	AGG	AGC	Arg	Ser	DMU	NCI-H324	34	92	Controversy with other publications
259	GAC	GTC	Asp	Val	DMU	NCI-H324	21	92	Controversy with other publications
158	CGC	CTC	Arg	Leu	SM	NCI-H441	92	92	Confirmed in another publication. wt in COSMIC
146	TGG	TGA	Trp	Stop	SM	NCI-H520	51	92	wt in COSMIC
191	CCT	del1a	Pro	Fs.	SM	NCI-H522	8	92	Single report
245	GGC	TGC	Gly	Cys	SM	NCI-H596	86	92	Confirmed in another publication
164	AAG	AAT	Lys	Asn	SM	NCI-H650	8	92	Single report
215	AGT	ATT	Ser	Ile	SM	NCI-H661	25	92	wt in COSMIC
217	GTG	TTG	Val	Leu	DMU	NCI-H676	3	92	Single report
248	CGG	CTG	Arg	Leu	DMU	NCI-H676	124	92	Single report
102	ACC	del1	Thr	Fs.	SM	NCI-H679	1	92	Single report
176	TGC	TGG	Cys	Trp	SM	NCI-H720	19	92	wt in COSMIC
162	ATC	ins9c	Ile	InF	SM	NCI-H727	1	1707	Single report
267	CGG	CCG	Arg	Pro	SM	NCI-H738	19	678	Single report
284	ACA	CCA	Thr	Pro	SM	NCI-H820	10	106	Single report
285	GAG	AAG	Glu	Lys	SM	NCI-H854	165	92	Single report
249	AGG	AGT	Arg	Ser	SM	OG56	389	1976	Single report
334	GGG	GTG	Gly	Val	SM	PC-1	3	1382	Single report
245	GGC	TGC	Gly	Cys	SM	PC-10	86	364	Single report
334	GGG	GTG	Gly	Val	SM	PC-13	3	1382	Single report
248	CGG	TGG	Arg	Trp	SM	PC-14	728	1382	Controversy with other publications
248	CGG	CAG	Arg	Gln	SM	PC-14	883	2242	Controversy with other publications
282	CGG	TGG	Arg	Trp	SM	PC-3	600	364	Confirmed in another publication
214	CAT	CGT	His	Arg	SM	PC-7	69	1382	Single report
248	CGG	CAG	Arg	Gln	SM	PC-9	883	364	Single report
104	CAG	TAG	Gln	Stop	SM	PERF-LC-AI	18	1976	Single report
244	GGC	TGC	Gly	Cys	SM	RAL	53	1393	Single report
113	TTC	TGC	Phe	Cys	SM	RERF-LOCK	9	1382	Single report
158	CGC	CTC	Arg	Leu	SM	SK-LC-6	92	1976	Single report
193	CAT	CGT	His	Arg	SM	SKLU1	86	16	Single report
280	AGA	AAA	Arg	Lys	SM	SK-MES-1	78	1081	Controversy with other publications. Excluded from the cosnensus
298	GAG	TAG	Glu	Stop	SM	SK-MES-1	71	303	Consensus based on three publications. Controversy with other publications
277	TGT	TTT	Cys	Phe	SM	SW1271	48	16	Single report
167	CAG	TAG	Gln	Stop	SM	SW900	40	2249	Mutation in COSMIC database
347	GCC	ACC	Ala	Thr	SM	U-1752	3	2021	Single report
175	CGC	del1	Arg	Fs.	SM	U-1810	3	2021	Single report
175	CGC	CAC	Arg	His	SM	VRMC-LCD	1187	1976	Single report
204	GAG	TAG	Glu	Stop	SM	Y-ML-1B	46	1867	Single report