U87 -tế bào u thần kinh đệm

From Wikipedia, the free encyclopedia

For one of the several German U-boats sometimes referred to as U87, see German submarine U-87.

In cell biology, U87 is a human primary glioblastoma cell line formally known as U-87 MG (abbreviation for Uppsala 87 Malignant Glioma). It has epithelial morphology, and was obtained from a stage four 44-year-old cancer patient.^[1] In 2016, the commonly used version of U87MG was found to be non-identical to its patient of origin.^[2]

U-87 MG can be obtained from the American Type Culture Collection (ATCC) where it is known by the accession number HTB-14.

The entire sequence of the genome of U-87 MG has been published in PLoS Genetics, 2010 January; 6(1): e1000832.

Growth conditions[edit]

U87 growth media is generally made with Eagle's minimum essential medium + 10% FBS + 100 U/ml penicillin + 100 ug/ml streptomycin.^[3] It is propagated at 37 degrees Celsius in a 5% carbon dioxide atmosphere.

References[edit]

1. Jump up^ "U87MG decoded: the genomic sequence of a cytogenetically aberrant human cancer cell line". PLoS Genet. 6 (1): e1000832. January 2010. doi:10.1371/journal.pgen.1000832. PMC 2813426. PMID 20126413.
2. Jump up^ "Origin of the U87MG glioma cell line: Good news and bad news.". Sci Transl Med. Aug 2016. PMID 27582061.
3. Jump up^ "HTB-14". ATCC.

External links[edit]

• ATCC record for HTB-14
• Cellosaurus entry for U87

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THP-1 (dòng tế bào monocytic)

THP-1 cell line-dòng tế bào monocytic

From Wikipedia, the free encyclopedia

THP-1 is a human monocytic cell line derived from an acute monocytic leukemia patient. It is used to test leukemia cell lines in immunocytochemical analysis of protein-protein interaction, and immunohistochemistry.

Contents

  [hide] 

• 1Cell Characteristics Growth Information
  • 1.1Characteristics
  • 1.2Growth Information
• 2References
• 3External links

Cell Characteristics Growth Information[edit]

Note that although THP-1 cells are of the same lineage, mutations can cause differences as the progeny proliferates.

Characteristics[edit]

These cells show a large, round, single-cell morphology.

They are derived from the peripheral blood of a 1 year old human male with acute monocytic leukemia. Some of their characteristics are:

• They have Fc receptor and C3b receptors and lack surface and cytoplasmic immunoglobulins.
• They produce IL-1.
• They stain positive for alpha-naphthyl butyrate esterase, produce lysozymes and are phagocytic (both for latex beads and sensitized erythrocytes).
• They can restore the response of purified T lymphocytes to Con A.
• They show increased CO₂ production on phagocytosis and can be differentiated into macrophage-like cells using for example phorbol 12-myristate 13-acetate(commonly known as PMA or TPA).
• The HLA type for THP-1 using molecular-based typing methods is HLA-A*02; B*15; C*03; DRB1*01, DRB1*15; DRB5*01/02;

DQB1*05, DQB1*06.

Growth Information[edit]

This cell line can provide continuous culture, grown in suspension; RPMI 1640 + 10% FBS + 2mM L-Glutamine. The average doubling time is 19 to 50 hours. You may also add 1 mM sodium pyruvate, penicillin (100 units/ml) and streptomycin (100 μg/ml) to inhibit bacterial contamination. Maintain cultures between 2-9x10⁵ cells/ml. 37C, 5% CO₂. Cells are non-adhesive.

Hazard: Cell line of human origin. There is no evidence for the presence of infectious viruses or toxic products. However, it is recommended that cultures are handled under Biosafety Level 2 containment.

References[edit]

• Tsuchiya S, Yamabe M, Yamaguchi Y, Kobayashi Y, Konno T, Tada K (August 1980). "Establishment and characterization of a human acute monocytic leukemia cell line (THP-1)". Int. J. Cancer. 26 (2): 171–6. doi:10.1002/ijc.2910260208. PMID 6970727.

External links[edit]

• Cellosaurus entry for THP-1

Saos-2 (tế bào Sarcoma xương)

Saos-2 cells-tế bào Sarcoma xương

From Wikipedia, the free encyclopedia

Saos-2 ("Sarcoma osteogenic") is a cell line derived from the primary osteosarcoma of an 11-year-old Caucasian girl in 1973 by Fogh et al.^[1]

In 1987 Rodan et al. determined that Saos-2 cells "possess several osteoblastic features and could be useful as a permanent line of human osteoblast-like cells and as a source of bone-related molecules."^[2]

Besides their worldwide availability, some of the advantages for using Saos-2 cell line are that they have well-documented characterization data, the possibility to obtain large amounts of cells in short time, and the fact that Saos-2 cells can be fully differentiated in a manner that the osteoblastic cells naturally do.^[3] The latter point is described particularly as "the ability of Saos-2 cells to deposit a mineralization-competent extracellular matrix",^[4] which makes these cells a valuable model for studying events associated with the late osteoblastic differentiation stage in human cells.^[5]

References[edit]

1. Jump up^ Fogh J, Fogh JM, Orfeo T (1977). "One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice". J Natl Cancer Inst. 59 (1): 221–226. PMID 327080.
2. Jump up^ Rodan SB, Imai Y, Thiede MA, Wesolowski G, Thompson D, Bar-Shavit Z, Shull S, Mann K, Rodan GA (1987). "Characterization of a human osteosarcoma cell line (Saos-2) with osteoblastic properties". Cancer Res. 47 (18): 4961–6. PMID 3040234.
3. Jump up^ Hausser HJ, Brenner RE (2005). "Phenotypic instability of Saos-2 cells in long-term culture". Biochem. Biophys. Res. Commun. 333 (1): 216–22. doi:10.1016/j.bbrc.2005.05.097. PMID 15939397.
4. Jump up^ McQuillan DJ, Richardson MD, Bateman JF (1995). "Matrix deposition by a calcifying human osteogenic sarcoma cell line (SAOS- 2)". Bone. 16 (4): 415–26. doi:10.1016/8756-3282(95)90186-8. PMID 7605701.
5. Jump up^ Gundle R, Beresford JN (1995). "The isolation and culture of cells from explants of human trabecular bone". Calcif. Tissue Int. 56 (Suppl 1): S8-10. PMID 7719993.

External links[edit]

• Information on SAOS-2 cells in the DSMZ database
• ATCC catalog
• Cellosaurus entry for Saos-2

MDA-MB-468-tế bào

From Wikipedia, the free encyclopedia

MDA-MB-468 is a cell line that was isolated from a 51 year old female human in 1977, and commonly used in cancer research. These cells were extracted from pleural effusion of mammary gland/breast tissue and have proven useful for the study of metastasis, migration and invasion of breast cancer.^[1] The cell line was isolated in 1977 by R. Cailleau, et al., from a pleural effusion of a 51-year-old Black female patient with metastatic adenocarcinoma of the breast. Although the tissue donor was heterozygous for the G6PD alleles, the cell line consistently showed only the G6PD A phenotype. ^[2]

See also[edit]

• MCF-7

KBM-7 (tế bào)

KBM-7 cells-tế bào

From Wikipedia, the free encyclopedia

KBM-7 cells are a Chronic myelogenous leukemia (CML) cell line used for biomedical research. Like all cancer cell lines, it is immortal and can divide indefinitely. A unique aspect of the KBM-7 cell line is that it is near-haploid, meaning it contains only one copy for most of its chromosomes.

Contents

  [hide] 

• 1Origin
• 2Cultivation
• 3References
• 4External links

Origin[edit]

KBM-7 cells were derived from a 39-year-old man with chronic myeloid leukemia in blast crisis.^[1] The original cell line contained both near haploid and hyperdiploid clones. Subsequent subcloning yielded a pure near-haploid cell line.^[2] Genome analysis has revealed that besides the disomic chromosome 8 also a 30 megabase fragment of chromosome 15 is present in two copies.^[3] Like other CML cells lines (e.g., K562) KBM-7 cells are positive for the Philadelphia chromosome harboring the BCR-ABL oncogenic fusion. KBM-7 cells have been reprogrammed to yield the HAP1 cell line which is also monosomy for chromosome 8.^[4]

Cultivation[edit]

KBM-7 cells grow in suspension and are maintained in Iscove's Modified Dulbecco's Medium (IMDM) supplemented with 10% fetal bovine serum. They divide approximately every 24 hours.

References[edit]

1. Jump up^ Andersson, B. S., Beran, M., Pathak, S., Goodacre, A., Barlogie, B., and McCredie, K. B. (1987). "Ph-positive chronic myeloid leukemia with near-haploid conversion in vivo and establishment of a continuously growing cell line with similar cytogenetic pattern". Cancer Genet. Cytogenet. 24: 335–343. doi:10.1016/0165-4608(87)90116-6.
2. Jump up^ Kotecki M, Reddy PS, Cochran BH (1999). "Isolation and characterization of a near-haploid human cell line". Exp Cell Res. 252 (2): 273–80. doi:10.1006/excr.1999.4656.
3. Jump up^ Bürckstümmer T, Banning C, Hainzl P, Schobesberger R, Kerzendorfer C, Pauler FM, Chen D, Them N, Schischlik F, Rebsamen M, Smida M, Fece de la Cruz F, Lapao A, Liszt M, Eizinger B, Guenzl PM, Blomen VA, Konopka T, Gapp B, Parapatics K, Maier B, Stöckl J, Fischl W, Salic S, Taba Casari MR, Knapp S, Bennett KL, Bock C, Colinge J, Kralovics R, Ammerer G, Casari G, Brummelkamp TR, Superti-Furga G, Nijman SM (2013). "A reversible gene trap collection empowers haploid genetics in human cells". Nat Methods. 10: 965–71. doi:10.1038/nmeth.2609. PMID 24161985.
4. Jump up^ Carette JE, Raaben M, Wong AC, Herbert AS, Obernosterer G, Mulherkar N, Kuehne AI, Kranzusch PJ, Griffin AM, Ruthel G, Dal Cin P, Dye JM, Whelan SP, Chandran K, Brummelkamp TR (2011). "Ebola virus entry requires the cholesterol transporter Niemann-Pick C1". Nature. 477 (7364): 340–3. doi:10.1038/nature10348. PMC 3175325. PMID 21866103.

External links[edit]

• Cellosaurus entry for KBM-7