Brain tumours

Table I : cell lines with wt p53

Cell line	ATCC	Origin	Reference
CHP-134*		Neuroblastoma
KG-1-C		Neuroblastoma	1006
GOTO		Neuroblastoma	1006
IMR-32*	CCL-127	Neuroblastoma
LAN-5*		Neuroblastoma
NB-1		Neuroblastoma	1006
NH-12		Neuroblastoma	1006
NH-6		Neuroblastoma	1006
SK-N-AS*	CRL-2137	Neuroblastoma
SH-SY5Y		Neuroblastoma
SK-N-SH*,**	HTB-11	Neuroblastoma
U-87-MG	HTB-14	Glioblastoma	2249
GM2300		Glioblastoma	1950
GM1578		Glioblastoma	1950
GM2455		Glioblastoma	1950
GM1600		Glioblastoma	1950
GM1592		Glioblastoma	1950
GM139		Glioblastoma	1950
GM2401		Glioblastoma	1950
SK-MG-11		Glioblastoma	698
SK-MG-15		Glioblastoma	698
V-MG-33		Glioblastoma	698

* Cytoplasmic p53
** a recent publication describes an abberant splice in this cell line leading to the expression of a 369 aa protein that lack the NLS (Nakamura Y, Ozaki T, Niizuma H, Ohira M, Kamijo T, Nakagawara A (2007) Functional characterization of a new p53 mutant generated by homozygous deletion in a neuroblastoma cell line. Biochem Biophys Res Commun 354: 892-898.)

Table II : cell lines with p53 gene deletion or rearrangement

Cell line	ATCC	Origin	Reference
LN-Z308		Glioblastoma

Table III : cell lines with p53 splice mutation
(exonic mutations that modify splice are listed in table IV)

No data available

Table IV: cell lines with p53 mutations (missense or frameshift)
More information is available in the handbook

Pos: Codon position (1 to 393);
WT: Normal base sequence of the codon in which the mutation occurred;
Mut Sequence of the mutated codon. If the mutation is a deletion or an insertion, it is indicated by "del" or "ins" followed by the number of deleted or inserted bases. The position in the codon is indicated by “a”, “b”, or “c” for the first, second or third base of the codon respectively. Example: "del66b" is a deletion of 66 bases including the second base of the codon; "ins4a" is an insertion of 4 bases occurring between the first and the second base of the codon. Label a, b, or c is omitted if the boundary of the deletion or insertion is unknown
AA: Wild type amino acid;
Mut: Mutant amino acid. Stop: nonsense mutation: Fs.: Frameshift mutation: InF: In-frame insertion or deletion;
Comp: complexity of the mutation: SM: Single mutational event in the tumor; DMU (Double Mutation Unknown): Two p53 mutations in the same tumor but their allelique distribution is unknown; DMD (Double Mutation Different allele): Two p53 mutations in the same tumor on two different p53 alleles; DMS (Double Mutation Same allele): Two p53 mutations in the same tumor on the same p53 allele: MM (Multiple Mutation): More than two p53 mutations in the same tumor;
NB: Number of tumors with this particular mutant in the database;
Ref: reference number;
Comments: information;

Table of references (available also in the handbook)

Astrocytoma
Pos.	WT	Mut	AA	Mut	Comp	Name	NB	Ref	Comments
115	CAT	TAT	His	Tyr	SM	440	2	491	Single report
55	ACT	ins1c	Thr	Fs.	SM	622	1	491	Single report
273	CGT	TGT	Arg	Cys	SM	8-MG-BA	687	2249	Mutation in COSMIC database
273	CGT	CAT	Arg	His	SM	B2-17	780	2249	Mutation in COSMIC database
248	CGG	TGG	Arg	Trp	SM	CAS-1	728	2249	Mutation in COSMIC database
248	CGG	TGG	Arg	Trp	SM	D-336MG	728	2249	Mutation in COSMIC database
245	GGC	AGC	Gly	Ser	SM	D-423MG	440	2249	Mutation in COSMIC database
245	GGC	AGC	Gly	Ser	SM	D-566MG	440	2249	Mutation in COSMIC database
239	AAC	ins3b	Asn	InF	SM	GB-1	1	2249	Mutation in COSMIC database
236	TAC	TGC	Tyr	Cys	SM	GMS-10	75	2249	Mutation in COSMIC database
245	GGC	AGC	Gly	Ser	DMU	KINGS-1	440	2249	Mutation in COSMIC database
248	CGG	CAG	Arg	Gln	DMU	KINGS-1	883	2249	Mutation in COSMIC database
175	CGC	CAC	Arg	His	SM	LN-319	1187	277	Single report
282	CGG	TGG	Arg	Trp	SM	LN-405	600	2249	Mutation in COSMIC database
110	CGT	CCT	Arg	Pro	SM	MOG-G-CCM	11	2249	Mutation in COSMIC database
159	GCC	GTC	Ala	Val	SM	MOG-G-UVW	50	2249	Mutation in COSMIC database
245	GGC	AGC	Gly	Ser	SM	no-10	440	2249	Mutation in COSMIC database
273	CGT	TGT	Arg	Cys	SM	no-11	687	2249	Mutation in COSMIC database
273	CGT	TGT	Arg	Cys	SM	SW1088	687	2249	Mutation in COSMIC database
273	CGT	TAT	Arg	Tyr	SM	SW1783	3	2249	Mutation in COSMIC database
238	TGT	GGT	Cys	Gly	SM	TM-31	10	1760	Single report
Glioblastoma
Pos.	WT	Mut	AA	Mut	Comp	Name	NB	Ref	Comments
273	CGT	TGT	Arg	Cys	SM	SJ-G2	687	1190	Single report
88	GCC	del1b	Ala	Fs.	SM	SJ-G3	1	1190	Single report
248	CGG	CAG	Arg	Gln	SM	SJ-G5	883	1190	Single report
242	TGC	TTC	Cys	Phe	SM	A-172	88	1397	wt in another publication
175	CGC	CAC	Arg	His	SM	A-7	1187	474	Single report
132	AAG	ATG	Lys	Met	SM	D456	9	472	Single report
195	ATC	ACC	Ile	Thr	SM	G-1163GM	90	2106	Single report
267	CGG	TGG	Arg	Trp	SM	G-1187GM	37	2106	Single report
248	CGG	TGG	Arg	Trp	SM	G123	728	370	Single report
105	GGC	AGC	Gly	Ser	SM	G-1265GM	1	2106	Single report
151	CCC	TCC	Pro	Ser	SM	G-1301M	92	2106	Single report
176	TGC	TAC	Cys	Tyr	SM	G-210GM	88	2106	Single report
179	CAT	GAT	His	Asp	SM	G-211GM	19	2106	Single report
249	AGG	ATG	Arg	Met	SM	G-599GM	64	2106	Single report
175	CGC	CAC	Arg	His	SM	G-750GM	1187	2106	Single report
175	CGC	CAC	Arg	His	SM	GM133	1187	1950	Single report
76	GCA	GGA	Ala	Gly	DMU	GM1596	9	1950	Single report
220	TAT	CAT	Tyr	His	DMU	GM1596	15	1950	Single report
163	TAC	TCC	Tyr	Ser	SM	GM2217	5	1950	Single report
248	CGG	TGG	Arg	Trp	SM	GM2313	728	1950	Single report
76	GCA	GGA	Ala	Gly	DMU	GM2328	9	1950	Single report
161	GCC	TCC	Ala	Ser	DMU	GM2328	5	1950	Single report
175	CGC	CAC	Arg	His	SM	GM2345	1187	1950	Single report
282	CGG	TGG	Arg	Trp	SM	GM2493	600	1950	Single report
237	ATG	ATA	Met	Ile	SM	GM47.23	123	987	Single report
175	CGC	CAC	Arg	His	SM	GM97	1187	1950	Single report
249	AGG	AGT	Arg	Ser	SM	GT9	389	1066	Single report
250	CCC	GCC	Pro	Ala	SM	GT9	14	1066	Single report
242	TGC	TTC	Cys	Phe	SM	LG	88	1397	Single report
238	TGT	TCT	Cys	Ser	SM	LN-18	10	277	Single report
164	AAG	GAG	Lys	Glu	SM	LN-229	25	277	Single report
197	GTG	CTG	Val	Leu	SM	LN382	2	2254	Single report
173	GTG	ATG	Val	Met	DMU	LN-428	77	277	Single report
282	CGG	TGG	Arg	Trp	DMU	LN-428	600	277	Single report
273	CGT	TGT	Arg	Cys	SM	SK-D1	687	698	Single report
173	GTG	CTG	Val	Leu	SM	SK-MG-16	23	698	Single report
255	ATC	ATT	Ile	Ile	SM	SK-MG-21	5	698	Single report
255	ATC	ATG	Ile	Met	SM	SK-MG-8	3	698	Single report
273	CGT	CAT	Arg	His	SM	SNB19	780	632	Confirmed in three other publications
237	ATG	ATA	Met	Ile	SM	T98G	123	277	Confirmed in two other publications. wt in COSMIC
213	CGA	CAA	Arg	Gln	SM	U118-MG	38	472	wt in COSMIC
232	ATC	ACC	Ile	Thr	DMU	U-138MG	15	1397	Single report
242	TGC	TTC	Cys	Phe	DMU	U-138MG	88	1397	Single report
273	CGT	CAT	Arg	His	SM	U251MG	780	2254	Single report
273	CGT	TGT	Arg	Cys	SM	V-MG-35/CE	687	698	Single report
218	GTG	GCG	Val	Ala	SM	V-MG-6	6	698	Single report
245	GGC	AGC	Gly	Ser	SM	D-542MG	440	2249	Mutation in COSMIC database
265	CTG	CCG	Leu	Pro	SM	GAMG	23	2249	Mutation in COSMIC database
241	TCC	TTC	Ser	Phe	SM	KALS-1	101	2249	Mutation in COSMIC database
342	CGA	TGA	Arg	Stop	SM	KNS-42	74	2249	Mutation in COSMIC database
286	GAA	AAA	Glu	Lys	SM	MO59J	86	1471	Single report
286	GAA	AAA	Glu	Lys	SM	MO59K	86	1471	Single report
266	GGA	GAA	Gly	Glu	SM	SF188	74	664	Single report
176	TGC	TAC	Cys	Tyr	SM	SF210	88	664	Single report
273	CGT	CAT	Arg	His	SM	SF268	780	664	Single report
248	CGG	CAG	Arg	Gln	SM	SF295	883	1018	Confirmed in COSMIC database
342	CGA	del1a	Arg	Fs.	SM	SF-539	5	2249	Controversy with other publications
258	GAA	AAA	Glu	Lys	SM	SNB75	73	1018	Confirmed in COSMIC database
275	TGT	TAT	Cys	Tyr	SM	GI-1	87	2249	Mutation in COSMIC database
Neuroblastoma
Pos.	WT	Mut	AA	Mut	Comp	Name	NB	Ref	Comments
113	TTC	TCC	Phe	Ser	SM	ACN	3	2249	Mutation in COSMIC database
342	CGA	CTA	Arg	Leu	SM	CHLA-119	1	1774	Single report
286	GAA	AAA	Glu	Lys	SM	CHLA-172	86	1774	Single report
286	GAA	AAA	Glu	Lys	SM	CHLA-90	86	1774	Single report
182	TGC	TGA	Cys	Stop	SM	LAN1	6	93	Single report
173	GTG	ATG	Val	Met	SM	NB13	77	2249	Mutation in COSMIC database
175	CGC	CAC	Arg	His	SM	NB16	1187	2249	Mutation in COSMIC database
177	CCC	ACC	Pro	Thr	SM	NB19	1	2249	Mutation in COSMIC database
176	TGC	TTC	Cys	Phe	SM	NB-SD	191	1190	Single report
245	GGC	AGC	Gly	Ser	SM	NMB	440	1772	Single report
176	TGC	TTC	Cys	Phe	SM	SJNB-4	191	1772	Single report
135	TGC	TTC	Cys	Phe	SM	SK-N-BE(2)	52	1772	Single report
110	CGT	CTT	Arg	Leu	SM	SK-N-DZ	28	2249	Mutation in COSMIC database
246	ATG	AGG	Met	Arg	SM	SK-N-FI	13	2249	Mutation in COSMIC database
282	CGG	del3a	Arg	InF	SM	TGW	1	2096	Single report
233	CAC	ins4b	His	Fs.	SM	CHP-100	1	93	Single report
PNET
Pos.	WT	Mut	AA	Mut	Comp	Name	NB	Ref	Comments
273	CGT	CAT	Arg	His	SM	HS-Sch-2	780	2078	Single report

Bladder carcinoma Table I : cell lines with wt p53 Cell line ATCC Reference BC16   729 HU456   729 HT1197*   729 KK47   729 RT4   561 PSI*   729 RT112   729 TCCSUP   729 UCRU-BL13     UCRU-BL-28     MGH-U     UM-UC2     UM-UC11     UM-UC6     mdm2 overexpression Table II : cell lines with p53 gene deletion or rearrangement No data Table III : cell lines with p53 splice mutation (exonic mutations that modify splice are listed in table IV) No data Table IV: cell lines with p53 mutations (missense or frameshift) More information is available in the handbook Pos: Codon position (1 to 393); WT: Normal base sequence of the codon in which the mutation occurred; Mut Sequence of the mutated codon. If the mutation is a deletion or an insertion, it is indicated by "del" or "ins" followed by the number of deleted or inserted bases. The position in the codon is indicated by “a”, “b”, or “c” for the first, second or third base of the codon respectively. Example: "del66b" is a deletion of 66 bases including the second base of the codon; "ins4a" is an insertion of 4 bases occurring between the first and the second base of the codon. Label a, b, or c is omitted if the boundary of the deletion or insertion is unknown AA: Wild type amino acid; Mut: Mutant amino acid. Stop: nonsense mutation: Fs.: Frameshift mutation: InF: In-frame insertion or deletion; Comp: complexity of the mutation: SM: Single mutational event in the tumor; DMU (Double Mutation Unknown): Two p53 mutations in the same tumor but their allelique distribution is unknown; DMD (Double Mutation Different allele): Two p53 mutations in the same tumor on two different p53 alleles; DMS (Double Mutation Same allele): Two p53 mutations in the same tumor on the same p53 allele: MM (Multiple Mutation): More than two p53 mutations in the same tumor; NB: Number of tumors with this particular mutant in the database; Ref: reference number; Comments: information; Table of references (available also in the handbook) Pos. WT Mut       AA Mut Comp Name NB Ref Comments 280 AGA ACA Arg Thr SM 5637 92 561 Confirmed in three other publications 248 CGG CAG Arg Gln SM 639-V 883 689 Single report 162 ATC AAC Ile Asn DMU 647-V 4 2249 Mutation in COSMIC database 336 GAG TAG Glu Stop DMU 647-V 5 2249 Mutation in COSMIC database 241 TCC TGC Ser Cys SM BFTC-909 36 564 wt in COSMIC 280 AGA ACA Arg Thr SM BL-17 92 859 Single report 126 TAC TAG Tyr Stop SM BT-1 14 689 Single report 241 TCC TTC Ser Phe SM CUBIII 101 729 Single report 158 CGC CAC Arg His SM DSH1 105 2249 Mutation in COSMIC database 126 TAC TAG Tyr Stop SM EJ 14 689 Controversy with other publications 164 AAG GAG Lys Glu SM EJ 25 729 Controversy with other publications 76 GCA del76 Ala Fs. SM FHS 738B1 1 561 Single report 365 CAC CGC His Arg SM HT-1197 1 561 wt in COSMIC 250 CCC CTC Pro Leu SM HT-1376 53 561 Confirmed in two other publications 261 AGT del137 Ser Fs. DMU J82 1 2249 Controversy with other publications Deletion of exon 8 271 GAG AAG Glu Lys MM J82 38 561 Controversy with other publications undocumented 4th mutation (deletion 210 bp) 271 GAG AAG Glu Lys MM J82 38 729 Controversy with other publications 274 GTT TTT Val Phe MM J82 32 561 Controversy with other publications undocumented 4th mutation (deletion 210 bp) 274 GTT TTT Val Phe MM J82 32 729 Controversy with other publications 320 AAG AAC Lys Asn MM J82 5 561 Controversy with other publications undocumented 4th mutation (deletion 210 bp) 320 AAG AAC Lys Asn DMU J82 5 2249 Controversy with other publications 320 AAG AAC Lys Asn MM J82 5 729 Controversy with other publications 241 TCC TAC Ser Tyr SM LB831-BLC 19 2249 Mutation in COSMIC database 213 CGA TGA Arg Stop SM LD137 306 1163 Single report 219 CCC del1a Pro Fs. SM LD600 10 1163 Single report 36 CCG CCA Pro Pro SM LD605 5 1163 Single report 245 GGC GAC Gly Asp SM LD627 171 1163 Single report 280 AGA AAA Arg Lys SM LD630 78 1163 Single report 248 CGG CAG Arg Gln SM LD660 883 1163 Single report 248 CGG CAG Arg Gln SM LD692 883 1163 Single report 158 CGC CTC Arg Leu SM LD700 92 1163 Single report 183 TCA TGA Ser Stop DMU RT-112 29 2249 Controversy with other publications 248 CGG CAG Arg Gln SM RT-112 883 1733 Controversy with other publications 248 CGG CAG Arg Gln DMU RT-112 883 2249 Controversy with other publications 110 CGT CTT Arg Leu SM SCaBER 28 294 Confirmed in another publication 110 CGT CTT Arg Leu SM SD 28 294 Controversy with other publications 116 TCT TGT Ser Cys SM SD 3 689 Controversy with other publications 273 CGT TGT Arg Cys SM SW1710 687 689 Single report 126 TAC TAG Tyr Stop SM T-24 14 689 Controversy with other publications 126 TAC del3a Tyr InF SM T-24 1 561 Controversy with other publications 349 GAA TAA Glu Stop SM TCCSUP 7 561 Single report 113 TTC TGC Phe Cys SM UM-UC-3 9 294 Confirmed in two other publications 126 TAC TAG Tyr Stop DMU VM-CUB-1 14 2249 Controversy with other publications 175 CGC CAC Arg His SM VM-CUB-1 1187 689 Controversy with other publications 175 CGC CAC Arg His DMU VM-CUB-1 1187 2249 Controversy with other publications 158 CGC CTC Arg Leu DMU VM-CUB-2 92 689 Controversy with other publications 158 CGC CTC Arg Leu SM VM-CUB-2 92 294 Controversy with other publications 163 TAC TGC Tyr Cys DMU VM-CUB-2 140 689 Controversy with other publications 278 CCT CTT Pro Leu SM VM-CUB-3 84 689 Single report

THE NCI-60 PANEL

Cell lines with an inconclusive p53 status are shown in red.

more information can be found here: Berglind et al., in press: download the manuscript

Cell line	ATCC number	DNA	Protein	ref	consensus	comment
LYMPHOMA LEUKEMIA
CCRF-CEM	CCL-119		R248Q	1018	R175H+R248Q	Two mutations in separate alleles
		c.524G>A, c.743G>A	R175H+R248Q	3
		c.524G>A, c.743G>A	R175H+R248Q	2249
HL-60	CCL-240		R248L	1018	p53 null
		null	p.M1_*394del	3000
		null	p.M1_*394del	2249
K-562	CCL-243	c.406_407insC	p.Q136fs*13	269	p.Q136fs*13	p53 RNA and protein are undetectable in this cell line
		c.406_407insC	p.Q136fs*13	2249
MOLT-4	CRL-1582	wt		3	Inconclusive	The status of MOLT-4 is highly heterogeneous in the literature. The report of a wt status could be due to the fact that only exons 5 to 8 (residues 126-306) were screened in several publications
		wt		1018
		c.331C>G	L111V	2242
		c.743G>A	R248Q	27
		c.916C>T	R306X	2249
RPMI-8226	CCL-155		R285L	1018	E285K	This mutant is temperature-sensitive
		c.853G>A	E285K	2249
		c.853G>A	E285K	98
SR	CRL-2262	wt		1018	wt
		wt		2249
NSCLC
A549	CCL-185	wt		1018	wt
		wt		16
		wt		2249
EKVX		del187-224 (exon 6)		1018	Inconclusive	Possible splicing defect. See below for more information
		c.609_610GG>TT	p.V203_E204>V	2249
HOP-62			Ins 212-225	1018		See below for more explanation
		c.673-2A>G  (splice junction)	p.?	2249
HOP-92			p.R175L	1018	R175L
		c.524G>T	p.R175L	2249
NCI-H226	CRL-5826		P309A	1018	Inconclusive
		c.473G>T	R158L	92
			wt	2249
NCI-H23	CRL-5800	c.738G>C	R246I	17	R246I
			R246I	1018
		c.738G>C	R246I	2249
NCI-H322M		c.743G>T	R248L	92	R248L
			R248L	1018
		c.743G>T	R248L	2249
NCI-H460	HTB-177	wt		1018	wt
		wt		2249
NCI-H522	CRL-5810		191delG	1018	p.P191fs*57
		c.572_572delC	p.P191fs*57	2249
		c.572_572delC	p.P191fs*58	92
COLON
COLO-205	CCL-222		G266E	1018	Inconclusive
		c.308_333>TA	p.Y103_L111>L	492
		c.308_333>TA	103del27	2249
HCC-2998			R213X	1018	R213X
		c.637C>T	R213X	2249
HCT-116	CCL-247	wt		1018	wt
		wt		2249
HCT-15	CCL-225	c.722C>T, c.1101-2A>C  (splice junction)	p.S241F, p.?	2249	Inconclusive
			P153A	1018
		c.722C>T	S241F	2251
HT-29	HTB-38	c.818G>A	R273H	492	R273H
		c.818G>A	R273H	2249
KM12			H179R	1018	Inconclusive
		c.215delG	p.R72fs*51	2249
SW620	CCL-227	c.818G>A, c.925C>T	R273H, P309S	9	R273H + P309S	SW480 and SW620 are derived from the same individual with a similar p53 alteration. The P309S mutation is not always reported
			R273H	1018
		c.818G>A, c.925C>T	R273H, P309S	2249
CENTRAL NERVOUS SYSTEN
SF268		c.818G>A	R273H	664	R273H
			R273H	1018
		c.818G>A	R273H	2249
SF295			p.R248Q	1018	R248Q
		c.743G>A	p.R248Q	2249
SF539		wt		1018	Inconclusive
		c.1024delC	p.R342fs*3	2249
SNB75			E258K	1018	E258K
		c.772G>A	E258K	2249
U251/SNB19		c.818G>A	R273H	632	R273H
			R273H	1018
		c.818G>A	R273H	2249
MELANOMA
LOXIMV1		wt		1018	wt
		wt		2249
Malme-3M	HTB-64	wt		1018	wt
		wt		2249
M14			G266E	1018	G266E
		c.797G>A	G266E	2249
SK-MEL-2	HTB-68		G245S	1018	G245S
		c.733G>A	G245S	2249
SK-MEL-28	HTB-72	c.434_435TG>GT	L145R	2249	L145R
		c.434T>G	L145R	1570
			C145V	1018
SK-MEL-5	HTB-70	wt		1018	wt
		wt		2249
UACC-257		wt		1018	wt
		wt		2249
UACC-62		wt		1018	wt
				2249
MDA-MB-435			G266E	1018	G266E	This cell line was originally reported as a breast carcinoma cell line but recent SNP analysis indicates that it is similar to the M14 melanoma cell line
		c.797G>A	G266E	2029
		c.797G>A	G266E	2249
MDA-N			G266E	1018	G266E	This cell line was originally reported as a breast carcinoma cell line but recent SNP analysis indicates that it is similar to the M14 melanoma cell line.  It is a derivative of MDA-MB-435 tranfected with a plasmid expressing erbB2
OVARY
IGROV1		wt		1018	Inconclusive
		wt		606
		c.377A>G	Y126C	2249
OVCAR-3	HTB-161	c.743G>A	R248Q	144	R248Q
			R248Q	1018
		c.743G>A	R248Q	2249
OVCAR-4		wt		1018	Inconclusive
		c.388C>G	L130V	2249
OVCAR-5			ins224	1018	Inconclusive
		wt		2249
OVCAR-8		c.376-1G>A		2249	Splicing defect	See below for more explanation
			del 126-132	1018
NCI/ADR-RES		c.376-1G>A		2249	Splicing defect	Originally named MCF-7/AdrR cells,  later re-designated NCI/ADR-RES.  Was recently found to be identical to OVCAR-8
			del 126-132	1018
			126del21	983
SK-OV-3	HTB-77		H179R	1018	Inconclusive	p53 RNA and protein are undetectable in this cell line: see below for more explanation
		c.267delC	p.S90fs*33	2249
RENAL
786-0	CRL-1932		P278A	1018	Inconclusive
		c.832C>G, c.560-2A>G  (splice junction)	p.P278A, p.?	2249
A498	HTB-44	wt		1018	wt
		wt		2249
ACHN	CRL-1611	wt		1018	wt
		wt		2249
CAKI-1	HTB-46	wt		1018	wt
		wt		2249
RXF393			R175H	1018	R175H
		c.524G>A	R175H	2249
SN12C			E336X	1018	E336X
		c.1006G>T	E336X	2249
TK10			L264R	1018	L264R
		c.791T>G	L264R	2249
U031		wt		1018	wt
		wt		2249
PROSTATE
DU-145	HTB-81		P223L	1018	P223L + V274F	See below for more explanation
		c.820G>T	V274F	2249
		c.668C>T + c.820G>T	P223L + V274F	3001
		c.668C>T + c.820G>T	P223L + V274F	59
PC-3	CRL-1435	c.414delC	p.K139fs*31	2249	p53 RNA and protein are undetectable in this cell line
			138del	1018
		c.414delC	138del	59
BREAST
BT-549	HTB-122	c.747G>C	R249S	24	R249S
			R249S	1018
		c.747G>C	R249S	2249
Hs 578T	HTB-126	c.469G>T	V157F	76	V157F
		c.469G>T	V157F	2249
			D157E	1018
MCF7	HTB-22	wt		1018	wt
		wt		249
MDA-MB-231	HTB-26	c.839G>A	R280K	24	R280K
			R280K	1018
T47D	HTB-133	c.580C>T	L194F	9	L194F
		c.580C>T	L194F	1018

In EKVX, the deletion of codon 187 to 224 detected on RNA-based analysis (ref 1018) corresponds exactly to the deletion of the entire exon 6, a strong argument for a splicing defect. Genomic analysis did not reveal a splicing defect but a tandem mutation at codons 203 and 204 in exon 6 (ref 2049). If the two cell lines analysed were really EKVX, this result suggests that a mutation at either codon 203 and/or 204 could affect p53 gene splicing

In OVCAR-8, the 126-132 deletion detected by the RNA-based assay (ref 1018) concerns the first six residues of exon 5. Genomic analysis (ref 2049) described a mutation in the acceptor site of exon 5 and a splicing defect leading to a shift of the normal donor site of exon 5 that skips 18 nucleotides (6 aa residues) in exon 5. Examination of the DNA sequence at codon 132 reveals an AG dinucleotide sequence preceded by a pyrimidine tract similar to those found in the splice donor sequence. The same situation is observed for NCI/ADR- RES that has been recently shown to be an ovarian carcinoma cell line originating from the same patient as OVCAR-8.

In HOP-62, RNA-based analysis (ref 1018) detected an insertion between codon 212-225 but no information about the insertion sequence was available. Codon 225 is at the boundary of exon 6 and intron 6 suggesting a splicing defect, as analysis at the genomic level (ref 2049) confirms the presence of a splice mutation in the acceptor signal of exon 6

In the majority of publications, the p53 status of the SK-OV-3 cell line is stated as “p53 null”. In fact, close examination of the original manuscript shows that the p53 gene in SK-OV-3 is not deleted and did not sustain any gross rearrangement but neither p53 RNA or protein are found. In these publications, no p53 mutations were found but the recent analysis performed at the Sanger Institute detected a deletion of a single nucleotide at position 267 (codon 90) (Ikediobi et al., 2006). It is therefore possible that nonsense-mediated mRNA decay (NMD) eliminates p53 aberrant mRNA. NMD has been observed in the human leukaemia cell line K562 where p53 is also inactivated via a 1 base pair insertion at nucleotide 136