Monday, 12 December 2016

KBM-7 (tế bào)

KBM-7 cells-tế bào

From Wikipedia, the free encyclopedia
KBM-7 cells are a Chronic myelogenous leukemia (CML) cell line used for biomedical research. Like all cancer cell lines, it is immortal and can divide indefinitely. A unique aspect of the KBM-7 cell line is that it is near-haploid, meaning it contains only one copy for most of its chromosomes.

Origin[edit]

KBM-7 cells were derived from a 39-year-old man with chronic myeloid leukemia in blast crisis.[1] The original cell line contained both near haploid and hyperdiploid clones. Subsequent subcloning yielded a pure near-haploid cell line.[2] Genome analysis has revealed that besides the disomic chromosome 8 also a 30 megabase fragment of chromosome 15 is present in two copies.[3] Like other CML cells lines (e.g., K562) KBM-7 cells are positive for the Philadelphia chromosome harboring the BCR-ABL oncogenic fusion. KBM-7 cells have been reprogrammed to yield the HAP1 cell line which is also monosomy for chromosome 8.[4]

Cultivation[edit]

KBM-7 cells grow in suspension and are maintained in Iscove's Modified Dulbecco's Medium (IMDM) supplemented with 10% fetal bovine serum. They divide approximately every 24 hours.

References[edit]

  1. Jump up^ Andersson, B. S., Beran, M., Pathak, S., Goodacre, A., Barlogie, B., and McCredie, K. B. (1987). "Ph-positive chronic myeloid leukemia with near-haploid conversion in vivo and establishment of a continuously growing cell line with similar cytogenetic pattern". Cancer Genet. Cytogenet. 24: 335–343. doi:10.1016/0165-4608(87)90116-6.
  2. Jump up^ Kotecki M, Reddy PS, Cochran BH (1999). "Isolation and characterization of a near-haploid human cell line". Exp Cell Res. 252 (2): 273–80. doi:10.1006/excr.1999.4656.
  3. Jump up^ Bürckstümmer T, Banning C, Hainzl P, Schobesberger R, Kerzendorfer C, Pauler FM, Chen D, Them N, Schischlik F, Rebsamen M, Smida M, Fece de la Cruz F, Lapao A, Liszt M, Eizinger B, Guenzl PM, Blomen VA, Konopka T, Gapp B, Parapatics K, Maier B, Stöckl J, Fischl W, Salic S, Taba Casari MR, Knapp S, Bennett KL, Bock C, Colinge J, Kralovics R, Ammerer G, Casari G, Brummelkamp TR, Superti-Furga G, Nijman SM (2013). "A reversible gene trap collection empowers haploid genetics in human cells". Nat Methods. 10: 965–71. doi:10.1038/nmeth.2609. PMID 24161985.
  4. Jump up^ Carette JE, Raaben M, Wong AC, Herbert AS, Obernosterer G, Mulherkar N, Kuehne AI, Kranzusch PJ, Griffin AM, Ruthel G, Dal Cin P, Dye JM, Whelan SP, Chandran K, Brummelkamp TR (2011). "Ebola virus entry requires the cholesterol transporter Niemann-Pick C1". Nature. 477 (7364): 340–3. doi:10.1038/nature10348. PMC 3175325Freely accessible. PMID 21866103.

External links[edit]

Posted by at No comments:

các dòng tế bào ung thư vú

List of breast cancer cell lines

From Wikipedia, the free encyclopedia
Scientists study the behaviour of isolated cells grown in the laboratory for insights into how cells function in the body in health and disease. Experiments using cell culture are used for developing new diagnostic tests and new treatments for diseases. This is a list of major breast cancer cell lines that are primarily used in breast cancer research.[Notes 1]

List of cell lines[edit]

Cell linePrimary tumorOrigin of cellsEstrogen receptorsProgesterone receptorsERBB2amplificationMutated TP53[Notes 2]Tumorigenic in miceReferenceExternal links
600MPEInvasive ductal carcinoma+––[3]Cellosaurus
AU565Adenocarcinoma––+–[3]Cellosaurus
BT-20Invasive ductal carcinomaPrimaryNoNoNoYesYes[4]Cellosaurus
BT-474Invasive ductal carcinomaPrimaryYesYesYesYesYes[5]Cellosaurus
BT-483Invasive ductal carcinoma++–[3]Cellosaurus
BT-549Invasive ductal carcinoma––+[3]Cellosaurus
Evsa-TInvasive ductal carcinoma, mucin-producing, signet-ring typeMetastasis(ascites)NoYes ?Yes ?[6]Cellosaurus
Hs578TCarcinosarcomaPrimaryNoNoNoYesNo[7]Cellosaurus
MCF-7Invasive ductal carcinomaMetastasis(pleural effusion)YesYesNoNo (wild-type)Yes (with estrogen supplementation)[8]Cellosaurus
MDA-MB-231Invasive ductal carcinomaMetastasis(pleural effusion)NoNoNoYesYes[9]Cellosaurus
SkBr3Invasive ductal carcinomaMetastasis(pleural effusion)NoNoYesYesNo[10]Cellosaurus
T-47DInvasive ductal carcinomaMetastasis(pleural effusion)YesYesNoYesYes (with estrogen supplementation)[11]Cellosaurus

Notes[edit]

  1. Jump up^ The original list was mainly based on the work of Lacroix and Leclercq (2004).[1]
  2. Jump up^ For more data on the nature of TP53 mutations in breast cancer cell lines, see Lacroix et al. (2006).[2]

References[edit]

  1. Jump up^ Lacroix M, Leclercq G (2004). "Relevance of breast cancer cell lines as models for breast tumours: an update". Breast Cancer Res Treat. 83 (3): 249–289. doi:10.1023/B:BREA.0000014042.54925.cc. PMID 14758095.
  2. Jump up^ Lacroix M, Toillon RA, Leclercq G (2006). "p53 and breast cancer, an update". Endocrine-related cancer. Bioscientifica. 13 (2): 293–325. doi:10.1677/erc.1.01172. PMID 16728565.
  3. ^ Jump up to:a b c d Neve RM, et al. (2006). "A collection of breast cancer cell lines for the study of functionally distinct cancer subtypes". Cancer Cell. 10 (6): 515–527. doi:10.1016/j.ccr.2006.10.008. PMC 2730521Freely accessible. PMID 17157791.
  4. Jump up^ Lasfargues EY, Ozzello L (1958). "Cultivation of human breast carcinomas". Journal of the National Cancer Institute. 21 (6): 1131–1147. PMID 13611537.
  5. Jump up^ Lasfargues EY, Coutinho WG, Redfield ES (1978). "Isolation of two human tumor epithelial cell lines from solid breast carcinomas". Journal of the National Cancer Institute. 61(4): 967–978. PMID 212572.
  6. Jump up^ Borras M, Lacroix M (1997). "Estrogen receptor-negative/progesterone receptor-positive Evsa-T mammary tumor cells: a model for assessing the biological property of this peculiar phenotype of breast cancers". Cancer Letters. 120 (1): 23–30. doi:10.1016/S0304-3835(97)00285-1. PMID 9570382.
  7. Jump up^ Hackett AJ, Smith HS, Springer EL, Owens RB, Nelson-Rees WA, Riggs JL, Gardner MB (1977). "Two syngeneic cell lines from human breast tissue: the aneuploid mammary epithelial (Hs578T) and the diploid myoepithelial (Hs578Bst) cell lines". Journal of the National Cancer Institute. 58 (6): 1795–1806. PMID 864756.
  8. Jump up^ Soule HD, Vazguez J, Long A, Albert S, Brennan M (1973). "A human cell line from a pleural effusion derived from a breast carcinoma". Journal of the National Cancer Institute. 51 (5): 1409–1416. PMID 4357757.
  9. Jump up^ Cailleau R, Young R, Olivé M, Reeves WJ Jr (1974). "Breast tumor cell lines from pleural effusions". Journal of the National Cancer Institute. 53 (3): 661–674. PMID 4412247.
  10. Jump up^ Engel LW, Young NA (1978). "Human breast carcinoma cells in continuous culture: a review". Cancer Research. 38 (11 Pt 2): 4327–4339. PMID 212193.
  11. Jump up^ Keydar I, Chen L, Karby S, Weiss FR, Delarea J, Radu M, Chaitcik S, Brenner HJ (1979). "Establishment and characterization of a cell line of human breast carcinoma origin". European Journal of Cancer. 15 (5): 659–670. doi:10.1016/0014-2964(79)90139-7. PMID 228940.
Posted by at No comments:
Subscribe to: Comments (Atom)